|
Session
|
Lecture
Topics
|
Laboratory
Experiences |
|
1
|
Nature of Disease
Infectious Diseases
HIV/AIDS
|
HIV/AIDS video
(Salk Institute)
HIV/AIDS Lab Exercise
|
|
2
|
Genetics
Mitosis/Meiosis
Human Development
Inheritable
Diseases
|
Make a baby - paper
Karyotyping
Fertilizing sea
urchin eggs
Mitosis/meiosis
lab - paper
|
|
3
|
Respiratory
and Cardiovascular
Heart and Vascular
Diseases
Lung Diseases
|
Physical heart/lung
tests
Lime-water lab
Water displacement
Making a plexiglass
lung
|
|
4
|
Digestion/Nutrition
Vitamins and
Minerals
E.coli and Other
Bacteria
"Natural
Foods" Issue
|
Digestion labs
Reading labels with Pyramids
Free resource offer
|
|
Session
|
Lecture
Topics
|
Laboratory
Experiences |
|
1
|
Pretest
Chemistry Review
Cells
|
Diffusion
and Osmosis |
|
2
|
Cells (continued)
Integument
|
Begin
Fetal Pig Dissection |
|
3
|
Skeletal
System |
Bones
and Joints |
|
4
|
Muscular
System |
Continue
Pig Dissection |
|
5
|
Nervous
System |
Set up Banana Lab
Neuromuscular Function
|
|
6
|
Heart
and Vessels |
Blood Pressure
Microcirculation
in Fish
|
|
7
|
Respiratory
Urinary (brief)
|
Respirometry |
|
8
|
Digestion
and Nutrition |
Observe Bananas
Digestion Lab
|
|
9
|
Reproduction and
Development
Birth Control
|
|
|
10
|
Post Test (mandatory
for program completion)
Cadaver Observation
|
|
|
BASIC
TECHNIQUES
|
|
Session
1
|
Project
1 (Lab. 1 in manual)
|
Measurements,
Micropipetting, and Sterile Techniques (3) |
|
|
|
|
Session
2
|
Project
2 (Lab. 2 in manual)
|
Bacterial Culture
Techniques (13)
A. Isolation of
Individual Colonies (14)
B. Overnight Suspension
Cultures (22)
C. Mid-Log Suspension
Culture (26)
|
|
|
PLASMID
RECOMBINATION AND IDENTIFICATION
|
|
Sessions
3 and 4
|
Project
3 (Lab. 7 in manual)
|
Recombination of
Antibiotic-Resistance Genes (111)
A. Restriction
Digest of Plasmids pAMP and pKAN (113)
B. Ligation of
pAMP and pKAN Restriction Fragments (120)
|
|
|
|
|
Session
5
|
Project
4 (Lab. 8 in manual)
|
Transformation
of E.coli with Recombinant DNA (125)
A. Classic Procedure
for Making Competent Cells (127)
B. Transformation
of E.coli with Recombinant pAMP/pKAN Plasmids (132)
|
|
|
|
|
Session
6
|
Project
5 (Lab. 9 in manual)
|
Replica
Plating to Identify Mixed E.coli Populations (141) |
|
|
|
|
Session
7
|
Project
6 (Lab. 10 in manual)
|
Purification and
Identification of Recombinant Plasmid DNA (149)
A. Plasmid Minipreparation
of pAMP/pKAN Recombinants (150)
B. Restriction
Analysis of Purified pAMP/pKAN Recombinants (155)
|
|
|
SOUTHERN
HYBRIDIZATION
|
|
Session
8
|
Project
7 (Lab. 13 in manual)
|
Southern Hybridization
of λDNA (91)
A. Restriction
and Electrophoresis of λDNA (193)
B. Southern Transfer
of λRestriction Fragments (200)
C. Hybridization
of the λProbe (206)
D. Nonradioactive
Probe Detection (210)
|
| Session
1 |
|
|
|
Lecture: |
General
overview |
|
|
Lifecycle
of flies |
|
|
Genetics |
|
|
|
|
Laboratory: |
Establish
cultures |
|
|
Examine
phenotypes |
|
|
Set
up single-trait genetic crosses |
|
|
|
| Session
2 |
|
|
|
Lecture: |
Drosophila
genetics II |
|
|
|
|
Laboratory: |
Analysis
of single-trait genetic cross |
|
|
Set
up double-trait and sex-linked genetic crosses |
|
|
|
| Session
3 |
|
|
|
Lecture: |
Drosophila
genome |
|
|
Genes
and chromosomes |
|
|
|
|
Laboratory: |
Analysis
of double-trait and sex-linked genetic crosses |
|
|
Chromosome
squashes |
|
|
|
| Session
4 |
|
|
|
Lecture: |
Web-based
sources for Drosophila and other organisms |
|
|
|
|
Laboratory: |
Computer
laboratory for Drosophila and genetic resources |
|
|
|
| Session
5 |
|
|
|
Lecture: |
Cloning
genes by computer |
|
|
|
|
Laboratory: |
Computer
laboratory for virtual cloning and analysis of genes |
|
|
|
| Session
6 |
|
|
|
Lecture: |
P-element
mutagenesis |
|
|
|
|
Laboratory: |
Assay
for reporter gene activity in mutant embryos |
|
|
|
| Session
7 |
|
|
|
Lecture: |
Motor
system function |
|
|
|
|
Laboratory: |
Analysis
of reporter gene expression in embryos |
|
|
Assay
for flight behavior |
|
|
|
| Session
8 |
|
|
|
Lecture: |
Olfactory
system function |
|
|
|
|
Laboratory: |
Assays
for olfactory behavior |
|
|
|
| Session
9 |
|
|
|
Lecture: |
Designing
experiments for science projects |
|
|
|
|
Laboratory: |
Designing
experiments for science projects |
|
|
|
| Session
10 |
|
|
|
Lecture/lab: |
Presentation
and critique of experiments
Course wrap-up |
