A brief description of the microwave-induced plasma emission detector for gas chromatography.
A real hypertextbook on the web. Great for anyone wanting to learn the basics of LC techniques! Requires a frame-compliant browser, such as Netscape 2.0.
A "task description" for a laboratory automation project for automated analysis and automated data interpretation of EPA Method 8080 for organochlorine compounds and polychlorinated biphenyls (PCBs).
Contains copies of the lecture presentations associated with a course in "Chemical Separations" by James K. Hardy at the University of Akron. Lecture slides can be viewed by selecting the appropriate chapter. This course stresses theory and application of modern chromatographic methods. Requires a frame-compliant browser, such as Netscape 2.0.
- Capillary column gas chromatography
- Chromatographic theory
- Distillation theory and practice
- Gas chromatography
- Hyphenated methods and interfacing
- Introduction to liquid chromatography
- Liquid chromatography equipment
- Multidimensional gas chromatography
- Qualitative evaluation of chromatographic data
- Quantitative analysis of chromatographic data
- Solvent extraction
- Supercritical fluid chromatography
- View animations associated with course
A brief description of chemiluminescence detection in HPLC using luminol chemistries.
This living document supports sampling and analytical activities for the evaluation of environmental and waste management samples from U.S. Department of Energy (DOE) sites. DOE Methods is the result of extensive cooperation from all DOE analytical laboratories. This document encompasses methods for collecting representative samples and for determining the radioisotope activity and organic and inorganic composition of a sample. The documents are in the .PDF format. You must have a copy of Adobe Acrobat® Reader or Exchange to view them. You can download Acrobat Reader for free from Adobe's World Wide Web server.
A set of laboratory instructions from the Lochmuller group.
The reason to use more than one kind of detector for gas chromatography is to achieve selective and/or highly sensitive detection of specific compounds encountered in particular chromatographic analyses. The determination of sulfur or phosphorus containing compounds is the job of the flame photometric detector.
The Biogeochemical Laboratories at Indiana University is the "birthplace" of Isotope Ratio Monitoring Gas Chromatography/ Combustion/ Mass Spectrometry. The main thrust of this Web page will be to service the GC/C/MS users community. Information on the technique, an extensive bibliography, software documentation and updates, and information on whom to contact for more information are all available at this Web location.
The simple geometry and small dimensions of the trapping medium in solid phase microextraction (coating on a fused silica fiber) enable very rapid desorption of the compounds extracted from a sample, which makes SPME a very good sample introduction technique for high-speed GC separations. A slide presentation by Janus Pawliszyn.
Intended to provide help for the students of chromatographic techniques and as support for advanced courses on high resolution chromatography. All other visitors are warmly welcome.
An Access Excellence science seminar background paper by Patrick McKay, senior research associate, Department of Recovery Sciences, Genentech, Inc. The page also contains instructions for a demonstration experiment, where Blue Dextran is mixed with a dilute Phenol Red solution in a buffer containing sodium chloride and sodium acetate at pH 5.0. At this pH, the phenol red turns yellow right in front of the students' eyes. Upon mixing with Blue Dextran, the solution turns green. Application of the solution to a Pharmacia PD-10 column separates the green back into blue and yellow.
Journal contents e-mail service. Clicking the link allows you to send a an e-mail, where the text subscribe separation-c should be the subject and the only contents of the e-mail body text. You'll get a reply back via e-mail to verify that your subscription has been properly entered. Make sure your e-mail parameters have been configured correctly if you are sending from a WWW browser.
A listing of titles of the symposium issues of J. Chromatogr. A.
Listing of monographies in the Elsevier Science Publishers title book series. A click on each entry leads to the table contents and ordering information.
Real-time, on-the-spot chemical sensing for industrial and government applications.
An index to the Chemical Science & Technology Laboratory of NIST, with pointers to small, nifty texts describing various techniques.
An organic chemistry resource developed by Yue-Ling Wong and Ching-Wan Yip, aimed to provide learning and teaching organic chemistry materials on the net. Includes MPEG and QuickTime movies on separatory funnel extraction, thin-layer chromatography with an interactive exercise and TLC Rf calculation, Gas chromatography, UV-Vis absorption spectroscopy, and NMR. Sections on distillation and filtration are not yet available. Also demonstrates multiple choice questions with instant feedback using the WWW forms feature.
A brief description of the gas chromatography detector based on ionization by deep UV light.
Adsorbent Resins are utilized by Purge and Trap (P&T) and other thermal desorption applications. These pages describe the use of breakthrough volume data on a number of adsorbent resins for more than 200 organic compounds as well as the use of this data and other adsorbent resin physical properties to determine the best adsorbent resin to select for your application.
SPME involves exposing a fused silica fibre that has been coated with a non-volatile polymeric liquid to a sample or its headspace. The absorbed analytes are thermally desorbed in the injector of a gas chromatograph (GC) or GC-mass spectrometer for separation and quantitation. A slide presentation by Janus Pawliszyn.
A popular article written by C.J. Welch, printed in Laboratory Equipment Digest in February 1995.
Thin-layer chromatography, abbreviated as TLC, is frequently employed for the qualitative analysis of mixtures and pure organic compounds. This link describes a TLC experiment.